Cholesterol efflux assay protocol
WebAug 13, 2024 · In some experiment, cellular cholesterol was trace-labeled using protocol described for cholesterol efflux, and the radioactivity in fractions were analyzed on a liquid scintillation counter. Lipidomics. ... WebSep 1, 2024 · Cholesterol efflux assay. Macrophage-specific efflux capacity was measured using BODIPY-cholesterol by a method reported previously with modifications . J774 mouse macrophages were dispensed into a 48-well plate at 7 × 10 4 cells per well. After 24 h, the cells were incubated for 2.5 h with 8 μmol/L BODIPY-cholesterol and in …
Cholesterol efflux assay protocol
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WebFluorescent-labelled cholesterol is a promising strategy to analyze and investigate the properties and metabolism of natural cholesterol in vitro. Its main advantages are that it can be taken up by cells, allows for intracellular and membrane distribution studies, and can be applied to cholesterol efflux assays such as in this protocol (Figure 7). WebCholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides a safe, sensitive, and reproducible method for measuring cholesterol efflux. ... Cholesterol Efflux Assay Protocol: The procedure described below is for macrophage cell line J774.1 ...
WebCholesterol Efflux Assay Protocol The procedure described below is for murine macrophage cell line J774.1. This procedure can also be used with macrophage cells derived from THP-1 monocytes, which can be differentiated into cells with a macrophage-like phenotype upon treatment with 100 nM phorbol 12-myristate-13-acetate (PMA) for … WebVII. Cholesterol Efflux Assay Protocol: The procedure described below is for murine macrophage cell line J774.1. This procedure can also be used with macrophage cells derived from THP-1 monocytes, which can be differentiated into cells with a macrophage-like phenotype upon treatment with 100 nM phorbol 12-myristate-13 …
WebThe Cholesterol Efflux Assay Kit is a high-throughput screening assay that measures cholesterol efflux in cells using fluorescent-labeled cholesterol (λ Ex = 482 nm/ λ Em = 515 nm). It is suitable for serum, isolated, or recombinant lipoprotein samples. WebMar 22, 2024 · The cholesterol efflux assay showed no difference between HBP treated and untreated with TNFα for 24 h in the presence of HDL and APOA-I ... The resazurin assay was performed based on the protocol of Jennings et al. . A resazurin stock solution at 880 µM (R7017, Sigma) was prepared by dissolving the powder in 0.1 N NaOH (0.011 …
WebJul 31, 2013 · an efficient and significantly shorter cholesterol efflux protocol that can be used for functional investigations in cultured MEC. Consequently, by applying this functional assay to MEC in the fleetwood mac number 1 singleWebDec 1, 2011 · Studies have shown a negative association between cellular cholesterol efflux and coronary artery disease (CAD). Standard protocol for quantitating cholesterol efflux involves labeling cells with [3 H]cholesterol and measuring release of the labeled sterol.Using [3 H]cholesterol is not ideal for the development of a high-throughput … chef ron back porch bessemerWebK603-100 is the same size as the 100 test size of ab65359. Other cholesterol assay kits include: - HDL and LDL/VLDL Cholesterol assay kit ab65390. - C ell-based Cholesterol assay kit ab133116. - Cholesterol … chef ron\\u0027s gumbo shopWebMar 6, 2012 · The cholesterol efflux assay is designed to quantitate the rate of cholesterol efflux from cultured cells. It measures the capacity of cells to maintain cholesterol efflux and/or the capacity of plasma acceptors to accept cholesterol released from cells. The assay consists of the following steps. che from sex and the cityWebThe cholesterol efflux assay is designed to quantitate the rate of cholesterol efflux from cultured cells. It measures the capacity of cells to maintain cholesterol efflux and/or the capacity of plasma acceptors to accept cholesterol released from cells. The assay consists of the following steps. chefron rosaWebSep 27, 2011 · The BODIPY-cholesterol efflux correlated significantly with preβ-1 (r(2) = 0.6) but not with total HDL-cholesterol. Reproducibility of the BODIPY-cholesterol efflux assay was excellent between ... chef ron\u0027s norwichWebBackground: Cholesterol efflux as a key event in reverse cholesterol transport (RCT) is considered now as both diagnostic tool and a promising target for the treatment of atherosclerosis. Radioactive in vitro cholesterol efflux assay (CEA) is the gold standard for determination of efflux at cellular level. Fluorescent tracers and stable isotope-labeled … chef ron\u0027s gumbo shop metairie