Fuheng biology shanghai china
WebNov 1, 2024 · Human embryonic lung fibroblast cell line MRC-5 was purchased from the cell bank of FuHeng Biology (Shanghai, China), maintained in MEM medium (Gibco, USA), supplemented with 10% FBS and 1% penicillin/streptomycin at 37 °C in a 5% CO 2 incubator. 2.2. Public datasets. WebHuman embryonic kidney HEK293T cells and mouse Leydig TM3 cells (FuHeng Biology, Shanghai, China; Cat# FH0244 and FH0337, respectively) were maintained in high-glucose DMEM medium (Gibco; Cat# C11960500BT) supplemented with 10% FBS and 1% penicillin-streptomycin at 37°C and 5% CO 2 in a humidified atmosphere.
Fuheng biology shanghai china
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WebNov 17, 2024 · Strains and growth conditions. L. reuteri SH 23 (DSMZ 8533) was cultured in MRS Broth at 37°C with shaking, and LEP expression was carried out using E. coli BL21(DE3) competent cells (Vazyme, Nanjing, China) and the pET-28a vector in our laboratory. HT-29 cells were purchased from FuHeng BioLogy (ShangHai, China) and … WebDec 5, 2024 · Cell culture. Human ESCC cell lines (KYSE150, EC9706, and ECA109) and HEK293 cells were purchased from Fuheng Biology (Shanghai, China). STR (short tandem repeat) amplification was used for cell ...
WebBiological Technology Co.Itd (Wuhan, China). Hepatic cell line (HL-7702 cells) was purchased from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Hepatocellular carcinoma cell line (SMMC-7721 cells) was got from Fuheng Biology (Shanghai, China). The water used in experiments was all Mill-Q doubly distilled water -1 WebJul 15, 2024 · For monocyte–ECs adhesion experiments, assays were performed by adding 5 × 10 5 THP1 cells (Zhong Qiao Xin Zhou Biotechnology Co., Ltd., Shanghai, China) that were labeled with 2 µM calcein-AM with green fluorescence (Beyotime Biotechnology, China) to 1 × 10 5 confluent HMEC-1 cells (FuHeng Biology, Shanghai, China) for 2 h …
WebApr 8, 2024 · State Key Laboratory of Rare Earth Resource Utilization and Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P. R. China WebJul 26, 2024 · L929 (a murine fibroblast cell line) and RAW cells (RAW 264.7, a murine-derived macrophage cell line) were purchased from the Cell Bank of Shanghai Institutes for Biological Sciences (Shanghai, China). Mouse artery endothelial cells (MAECs) were purchased from FuHeng Biology (Shanghai, China).
WebMar 14, 2024 · The LUAD cell line A549 (cat. no. FH0045) was obtained from the Cell Center of FuHeng Biology (Shanghai, China). Cells were grown in RPMI 1640 (Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Lonsera Science SRL) and penicillin (100 IU/ml)/streptomycin (100 µg/ml) (MedChemExpress), and in a humidified …
WebHepatic cell line (HL-7702 cells) was purchased from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Hepatocellular carcinoma cell line (SMMC-7721 cells) was … farlight stoneWebNov 26, 2024 · Three rounds of biopanning were performed to obtain the antigen-specific VHH fragments with naïve nanobody phage library (Naïve VHH library) prepared with … farlightstoneWebAug 13, 2024 · Besides, DF-1 cells (Fuheng biology, Shanghai, China) were cultured in growth medium. All medium was changed every 24 h. SMSCs were transfected by … farlim all seasonWebApr 25, 2024 · The MKN-45 cell line was purchased from FuHeng Biology (Shanghai, China). SGC-7901, MGC-803, and GES-1 cell lines were purchased from Zhong Qiao Xin Zhou Biotechnology Co., Ltd. (Shanghai, China). SGC-7901, MKN-45, MGC-803, and GES-1 cell lines were cultured in 10% FBS RPMI 1640 complete medium supplemented … far light sport aircraftWebTo study the role of cytidine-phosphate-guanosine oligodeoxynucleotide (CpG ODN) on the development of Plasmodium liver stage. Plasmodium yoelii BY265 18S rRNA was … farlim electrical shopWebJan 30, 2024 · Therefore, sex is one of the most important scientific topics and is the queen of questions in evolutionary biology. Sex determination is a key issue for managing … farlight shoesWebDec 3, 2024 · BV2 microglial cells (FuHeng Biology, Shanghai, China) were cultured in DMEM (Sigma-Aldrich) with 10% FBS (Sigma-Aldrich), 100 U/mL penicillin (Invitrogen, CA, USA), and 0.1 mg/mL streptomycin (Invitrogen, CA, USA) in a humidified atmosphere of 5% CO 2 and 95% air at 37 °C. free newspaper classified ads