2024 Pyrearinus termitilluminans

Pyrearinus termitilluminans

Author: uvwk

August undefined, 2024

WebCloser examination reveals that the tiny pinpoints of light are produced by the larvae of a beetle (Pyrearinus termitilluminans) with a luminous tip to its abdomen. Each termite mound has hundreds of larvae living in its outer skin. WebAug 31, 2024 · Similar results were observed in Pichia pastoris cells expressing Pyrearinus termitilluminans luciferase, but in this case the I C 50 was ~ 100 µM for p-benzoquinone (Supplementary Fig. S3).

Pyrearinus termitilluminans larval click beetle luciferase: active …

• Pyrearinus acutus (Candèze, 1863) Colombia • Pyrearinus adustus Costa, 1978 Brazil • Pyrearinus alvarengai (Cobos, 1959) Argentina; Brazil • Pyrearinus amplicollis (Candèze, 1863) French Guiana WebComparative multialignment and site-directed mutagenesis using different color emitting click-beetle luciferases from P. angustus, Fulgeochlizus bruchi and Pyrearinus termitilluminans luciferases cloned by our group showed an integral role of residue 247 in bioluminescence color modulation. marcelo lima vol 3

The complete mitochondrial genome of click beetle

WebPyrearinus termitilluminans (E-Luc) and Luciola curciata (Red-F) Insert Size (bp) 7071 Mutation. c.375C>T silent mutation to remove BbsI site (E-Luc), p.S286Y Red mutant (RedF) Promoter pEstradiol_Inducible Cloning Information ... WebTrypsin-like enzymes from the salivary gland complex (SGC) of Lygus hesperus Knight were partially purified by preparative isoelectric focusing (IEF). Enzyme active against Nα-benzoyl-l-arginine-p-nitroanilide (BAp NA) focused at approximately pH 10 during IEF.This alkaline fraction gave a single activity band when analyzed with casein zymograms. WebDec 9, 2008 · The evolutionary origin of beetle bioluminescence is enigmatic. Previously, weak luciferase activity was found in the non-bioluminescent larvae of Tenebrio molitor (Coleoptera: Tenebrionidae), but the detailed tissular origin and identity of the luciferase-like enzyme remained unknown. Using a closely related giant mealworm, Zophobas morio , … marcelo madalozzo

Luciferin biosynthesis in larval Pyrearinus termitilluminans ...

WebWe present a comprehensive phylogenetic analysis of Syntermitinae, including representatives of all genera of the subfamily, along with all 12 species assigned formerly to the genus Armitermes Wasmann (Termitidae, Syntermitinae), and 4 … WebFeb 25, 2011 · 2.1. Materials. The cDNA of click beetle (Brazilian Pyrearinus termitilluminans) luciferase (Emerald Luc; ELuc) and Emerald Luciferase Assay Reagent were obtained from Toyobo Co. Ltd. (Japan).The cDNA of railroad worm (Phrixotrix hirtus) luciferase (RWLuc) was a gift from Dr. Y. Ohmiya (AIST, Japan).The human cDNAs of … marcelo mabiliaWebDownload Table Summary of physical-chemical properties of Pyrearinus termitilluminans and other beetles luciferase Luciferase l max /nm (pH 8) K M /mM luciferin K M /mM ATP … csc volume 1 notes

"WebDec 1, 2009 · Several years ago we cloned Pyrearinus termitilluminans larval click beetle luciferase, which displays the most blue-shifted bioluminescence among beetle … " - Pyrearinus termitilluminans

Pyrearinus termitilluminans

Biosensing firefly luciferin synthesis in bacteria reveals a cysteine ...

WebThe luciferase and antioxidant enzyme levels were kept unaltered indicating constitutive levels of Pyrearinus termitilluminans larvae inside the termite mounds. In addition, concomitant hyperoxia and treatment with a luciferase inhibitor, namely the luciferin methyl ester (LME), increased prothorax SOD activity not above 30% along with higher levels of … WebPyrearinus termitilluminans (sp.n., Costa, 1982.) live in termite mounds in central Brazil. Each larva produces light in the segment immediately behind its head. Larvae were observed to luminesce only during the first weeks of the rainy season, the same times as the ant and termite alate flights.

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WebMay 15, 2012 · We co-cultured organ tissues from transgenic mice expressing the green-emitting beetle luciferase from Pyrearinus termitilluminans (ELuc, λ max = 538 nm) , under control of the mBmal1 promoter (Bmal1 ELuc mice) and Rat-1 fibroblasts expressing the red-emitting beetle luciferase from Phrixothrix hirtus (SLR, λ max = 630 nm) , under … WebSep 1, 2016 · Comparative multialignment and site-directed mutagenesis using different color emitting click-beetle luciferases from P. angustus, Fulgeochlizus bruchi and …

WebSep 1, 2010 · Quantum yield (QY) was significantly correlated with luminescence spectrum. The green light-emitting luciferase of the Brazilian click beetle, Pyrearinus termitilluminans, whose luminescence spectrum had the shortest peak wavelength of all the luciferases investigated, had the highest QY (0.61). WebThrough homology modeling and site-directed mutagenesis using our multicolor set of beetle luciferases (Pyrearinus termitilluminans larval click beetle, Pte, λmax = 534 nm; Phrixothrix hirtus railroad worm red emitting, PxRE, λmax = 623 nm; and Macrolampis sp2 firefly, Mac, λmax = 564 nm), we show that the residues C/T311 (S314) play an important …

WebQuantum yield (QY) was significantly correlated with luminescence spectrum. The green light-emitting luciferase of the Brazilian click beetle, Pyrearinus termitilluminans, whose luminescence spectrum had the shortest peak wavelength of all the luciferases investigated, had the highest QY (0.61). WebTo identify biologically relevant compounds in basic biology and drug discovery processes, rapid quantitative methods for elucidating protein−protein interactions have become necessary. We describe a novel optical technique for monitoring protein−protein interactions in living cells based on complementation of split luciferase fragments from click beetle …

WebNov 11, 2003 · Prey attraction as a possible function of bioluminescence in the larvae of Pyrearinus termitilluminans (Coleoptera: Elateridae) K. Redford; Biology. 1982; TLDR. The prey-catching and food-storing behavior and the phenomenon of bioluminescence are apparently an evolutionary response by P. termitilluminans larvae to a short, rich pulse ...

WebMar 9, 2024 · They used two luciferases: a green-emitting ELuc derived from Pyrearinus termitilluminans and a red-emitting SLR derived from Phrixothrix hirtus. The bioluminescences of single cells that were transfected with two mammalian clock-gene reporters showing different phases were separated into green- and red-color images … marcelo latinoWebOct 1, 2015 · A red-shifted variant of ELUC from Photinus termitilluminans (PtRLUC) was used for dual-color imaging. This ELUC variant carries two amino acid substitutions that … marcelo magnoWebModeling studies with Phrixothrix, Pyrearinus termitilluminans, and Macrolampis luciferases 4, 37 showed that the variable position represented by residues C311, T311, … marcelo longobardi ultimas noticiasWebPyrearinus termitilluminans is a species of insects with 5 observations marcelo mastroroccoWebJun 21, 2024 · Homology-based models of Phrixotrix hirtus, Pyrearinus termitilluminans, Amydetes vivianii and Macrolampis sp2. luciferases were constructed using as template … marcelo maestri oftalmologista porto alegreWebJul 1, 2016 · Abstract. Luminescent termite mounds caused by colonization of the luminescent larvae of the click beetle Pyrearinus termitilluminans (Costa, 1982) are … marcelo magnaniWebJan 20, 2015 · In this study, we identified a potent destabilization sequence (referred to as the C9 fragment) consisting of 42 amino acid residues from human calpain 3 (CAPN3). … marcelo incendios